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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: LncGBP9/miR-34a axis drives macrophages toward a phenotype conducive for spinal cord injury repair via STAT1/STAT6 and SOCS3

Fig. 5

LncGBP9 modulates SOCS3 through miR-34a in macrophages. a The expression of miR-34a determined in M0, M1, and M2 macrophages by real-time PCR. b The expression of miR-34a in response to lncGBP9 silencing determined in M1 macrophages by real-time PCR. c miR-34a overexpression conducted in M1 macrophages by transfection of miR-34a mimics, as confirmed by real-time PCR. d LncGBP9 expression in response to miR-34a overexpression determined in M1 macrophages by real-time PCR. e SOCS3 protein level in response to miR-34a overexpression determined in M1 macrophages by immunoblotting. f miR-34a expression in response to lncGBP9 overexpression determined in M2 macrophages by real-time PCR. g miR-34a inhibition conducted in M2 macrophages by transfection of miR-34a inhibitor, as confirmed by real-time PCR. h LncGBP9 expression in response to miR-34a inhibition determined in M2 macrophages by real-time PCR. i SOCS3 protein level in response to miR-34a inhibition determined in M2 macrophages by Immunoblotting. *P < 0.05, **P < 0.01. Values are mean ± S.D of n = 4 independent experiments

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