Fig. 6

LncGBP9 serves as a ceRNA for miR-34a to counteract miR-34a-mediated SOCS3 suppression. a, b Schematic diagrams showing the predicted binding site between miR-34a and lncGBP9 and between miR-34a and SOCS3. Wild- and mutant-type GBP9 and SOCS3 3′-UTR luciferase reporter vectors (wt-GBP9/SOCS3 3′-UTR or mut-GBP9/SOCS3 3′-UTR) were constructed and co-transfected in 293T cells with miR-34a mimics/inhibitor; the luciferase activity was determined. c Association of miR-34a and lncGBP9 with AGO2 in M1 macrophages. Detection of AGO2 and IgG using Immunoblotting assays. d RIP assay in M1 macrophages transfected with control miRNA (NC mimics) or miR-34a mimics followed by real-time PCR to detect GBP9 and miR-34a associated with AGO2. IgG was used as negative control. e RIP assay in M1 macrophages transfected with control vector (NC) or lncGBP9-overexpressing vector followed by real-time PCR to detect GBP9, SOCS3, and β-actin associated with AGO2. β-actin was used as negative control. *P < 0.05, **P < 0.01. Values are mean ± S.D of n = 3 independent experiments