Fig. 7

LncGBP9/miR-34a axis modulates macrophage polarization via affecting the balance of STAT1/STAT6. a, c, e M1 macrophages were co-transfected with Ad-sh-lncGBP9 and miR-34a inhibitor and examined for the mRNA expression and protein levels of STAT1, p-STAT1, SOCS3, iNOS, and CD16/32, and the production of IL-6 and IL-12. b, d, e M2 macrophages were co-transfected with Ad-lncGBP9 and miR-34a mimics and examined for the mRNA expression and protein levels of STAT6, p-STAT6, SOCS3, Arg1, and CD206, and the production of IL-10 and TGF-β1. *P < 0.05, **P < 0.01, compared to control group; #P < 0.05, ##P < 0.01, compared to Ad-sh-NC + miR-34a inhibitor or Ad-lnc-NC + miR-34a mimics group. Values are mean ± S.D of n = 3 independent experiments