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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: IL-33 and its decoy sST2 in patients with Alzheimer’s disease and mild cognitive impairment

Fig. 5

NF-kB nuclear translocation. a Representative images of NF-kB nuclear translocation in unstimulated (MED) and in LPS-primed and Aβ42 stimulated monocytes. First column = cells in brightfield (BF); second column = NF-kB-FITC fluorescence; third column = 7AAD fluorescence; fourth column = NF-kB and 7AAD merged fluorescence. Fourth column: the upper image showing the red nuclear image (7AAD staining) surrounded by the green cytoplasmic NF-kB staining indicates lack of nuclear translocation (similarity score = − 1.85); the lower image, where NF-kB and the nuclear dye are colocalized, indicates nuclear translocation (similarity score = + 2.3). b NF-κB/7-AAD similarity histograms indicating the percentage of cells in which NF-kB is untranslocated (negative similarity score) or translocated (positive similarity score) to the nucleus. ce NF-kB nuclear translocation in unstimulated (MED) and in LPS-primed and Aβ42 stimulated monocytes in the absence or in the presence of human recombinant IL-33 (10 ng/ml); data obtained with cells of representative AD (c), MCI (d), and HC (e) individuals are shown. Summary results are presented in the bar graphs. The boxes stretch from the 25° to the 75° percentile; the line across the boxes indicates the median values. Statistical significance is shown

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