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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Spinal cord NLRP1 inflammasome contributes to dry skin induced chronic itch in mice

Fig. 3

Nlrp1 knockdown inhibits AEW-induced scratching behavior. a The scheme of AAV-shRNA infusion and AEW-induced dry skin itch. b Fluorescence images that expressed AAV-Nlrp1a-shRNA-EGFP in the spinal cord 28 days after infusion. Scale bar = 200 μm for the sagittal section (left). Scale bar = 100 μm for the cross section (right). Western blotting results confirming the knockdown efficacy of AAV-Nlrp1a-shRNA, n = 3. c Statistical results showing Nlrp1 knockdown reduced AEW-induced increase in the number of spontaneous scratches, n = 10. d, e Representative HE staining images and statistical results showing Nlrp1 knockdown reduced AEW-induced increase in the thickness of nucleated epidermal layers, n = 10. Scale bar = 100 μm. fi Representative immunoreactive bands and statistical results showing Nlrp1 knockdown reduced AEW-induced increase in the expression of ASC, caspase-1, IL-1β, and IL-18. n = 6, Data are expressed as means ± SEM, *P < 0.05 and **P < 0.01 vs control group; #P < 0.05 vs AEW group

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