Fig. 6

APC366 reduced FJC-positive degenerating neurons at 7 days after ACA. a Representative FJC staining microphotographs. b Quantitative analyses of FJC-positive cells in (a) hippocampal subiculum, (b) CA1, and (c) CA2/3. Scale bar = 50 μm. ACA caused significant neuronal degeneration in the subiculum and CA1 regions. The activation of PAR-2 with AC55541 further exacerbated neuronal degeneration in rats subjected to ACA. APC366 at doses of 50 μg and 150 μg significantly reduced the number of FJC-positive cells in the subiculum and CA1 regions compared to the vehicle or AC55541-treated ACA rats. The tendency toward better treatment efficacy of 50 μg APC366 than 150 μg APC366 did not reach statistical significance. Data are expressed as mean ± SD. n = 6/group. ANOVA, Tukey. **p < 0.001 compared to sham, *p < 0.05 compared to sham, &&p < 0.001 compared to ACA + vehicle, &p < 0.05 compared to ACA + vehicle