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Table 2 The number and distribution of the animals included for the present study

From: Inhibition of mast cell tryptase attenuates neuroinflammation via PAR-2/p38/NFκB pathway following asphyxial cardiac arrest in rats

  Groups (n)Mortality (n)
Time course studyWB (6, 12, 24, and 72 h post-ROSC)Sham (n = 4)0
ACA (6 h, 12 h, 24 h, 72 h) (n = 16)3 (1 died at 12 h, 1 died at 15 h, and 1 died at 22 h post-ROSC)
Cellular localization (24 h post-ROSC)Sham (n = 1), ACA (n = 1)0
Toluidine blue staining (24 h post-ROSC)Sham (n = 1)0
ACA (n = 1)0
Short-term outcome study (up to 7-day post ROSC)Fluoro-Jade C stainingSham (n = 6)0
ACA + vehicle (n = 6)2 (1 at 24 h post-ROSC, 1 died at 48 h post-ROSC)
ACA + APC366 (50 μg) (n = 6)2 (1 died at 48 h post-ROSC, 1 died at 70 h post-ROSC)
ACA + APC366 (150) (n = 6)1 (died at 6 h post-ROSC)
ACA + AC55541 (30 μg) (n = 6)2 (1 died on 5th day post-ROSC, 1 died on 6th post-ROSC)
Long-term outcome study (30-day post-ROSC)Fluoro-Jade C stainingSham (n = 6)0
ACA + vehicle (n = 6)0
ACA + APC366 (50 μg) (n = 6)0
Mechanism study (24 h post-ROSC)Western blotSham (n = 6)0
ACA + vehicle (n = 6)0
ACA + APC366 (50 μg) (n = 6)0
ACA + AC55541 (30 μg) (n = 6)0
ACA + APC366 (50 μg) + AC55541 (30 μg) (n = 6)1 (died at 8 h post-ROSC)
ACA + AC55541 (30 μg) + SB203580 (300 μg) (n = 6)0
 Mass spectrometryAPC366 (n = 1)0
Total12010911
  1. ACA asphyxial cardiac arrest, h hours, ROSC return of spontaneous circulation