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Fig 3 | Journal of Neuroinflammation

Fig 3

From: MAPK signaling determines lysophosphatidic acid (LPA)-induced inflammation in microglia

Fig 3

MAPK activation controls LPA-induced transcription factor phosphorylation. a) Primary microglia cells, isolated from C57BL/6 J pups (P0-P4), were cultured in PDL-coated 12-well plates and serum-starved overnight. Cells were incubated with DMSO (vehicle, highest final concentration 0.01%) and DMSO plus LPA (1 μM) in the absence or presence of SP600125 (10 μM), SB203580 (10 μM), or PD98059 (10 μM) for the indicated time periods. Cell protein lysates were collected and phosphorylated, and total STAT1, STAT3, p65-NF-kB, and c-Jun proteins were detected by immunoblotting. Protein/loading control ratios were normalized to the ratio of unstimulated microglia and one representative blot for each protein is presented. b Densitometric analysis from four independent immunoblot experiments. Results are presented as mean values + SD. (*p < 0.05, **p < 0.01, ***p < 0.001 compared to DMSO-treated cells; #p < 0.05, ##p < 0.01, ###p < 0.001 each individual inhibitor compared to LPA-treated cells; two-way ANOVA with Bonferroni correction)

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