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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Immuno-metabolic impact of the multiple sclerosis patients’ sera on endothelial cells of the blood-brain barrier

Fig. 8

Biochemical pathways of brain ECs treated with RRMS sera and matched HD. a Immunoblot for hexokinase, aldolase and enolase-1 on hCMEC/D3 cells treated overnight with 20% HD or RRMS naïve-to-treatment sera. Total ERK 1/2 served as a loading control. One representative out of at least three independent experiments performed on 12 donors in total/group. b Densitometry quantification for hexokinase, aldolase and enolase-1 performed on all 10 donors in total/group. c Immunoblot for DLST and DLAT, on hCMEC/D3 cells treated overnight with 20% HD or RRMS naïve-to-treatment sera. Total ERK 1/2 served as a loading control. One representative out of at least three independent experiments performed on 12 donors in total/group. d Densitometry quantification of DLST and DLAT normalized on total ERK 1/2 performed on all 10 donors. Data are expressed as mean ± s.e.m. (statistical analysis by Mann-Whitney U test (two-tails) *P ≤ 0.05)

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