Fig. 1
From: Embryonic microglia influence developing hypothalamic glial populations
Microglia expand their population during tuberal hypothalamic embryonic development. a Representative Iba1 immunostained images of coronal embryonic brain sections within the tuberal hypothalamus at E11.5, E13.5, E15.5, and E17.5 time points. Box and Whisker plots: b Total number of Iba1+ microglia within the entire embryonic tuberal hypothalamic area (mean = 339.9 ± 148.2, 965.9 ± 332.0, 1983 ± 599.8, 3845 ± 221.9 cells for E11.5, E13.5, E15.5, and E17.5, respectively; P < 0.0001) and (c) Density of Iba1+ microglia within the embryonic tuberal hypothalamus (mean = 3335.1 ± 470.6, 3021.6 ± 434.3, 3250.9 ± 410.1, 2875.5 ± 160.0 cells/mm3 for E11.5, E13.5, E15.5, and E17.5, respectively; P = 0.4616). Samples: n = 6, 5, 4, 4 embryos for E11.5, E13.5, E15.5, and E17.5, respectively. Graphs: Box and Whisker plots with middle line representing median, cross representing mean, box extending from the 25th to 75th percentiles, and whiskers at max and min values. Statistics: ANOVA with Tukey post hoc for multiple comparisons, *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001