Fig. 5

A subset of the Olig2+ cell population in the tuberal hypothalamus is altered in the PLX5622 microglia knock down model. Cell counts from immunostaining in control and PLX5622 treated embryonic and postnatal coronal brain slices in the rostral-to-mid tuberal hypothalamus. a Representative images of tuberal hypothalamus immunostained with Olig2/PdgfRα at E15.5, E17.5, and P2 in control and the PLX5622 microglia knock down model. b Olig2+/PdgfRα+ cells located within the MZ at E15.5 (P = 0.8264), E17.5 (P = 0.2609), and P2 (P = 0.7102). c Olig2+/PdgfRα− cells located adjacent to the ventricle in the VZ region at E15.5 (P = 0.3322) and E17.5 (P = 0.0457). d Olig2+/PdgfRα− cells located within the MZ at E15.5 (P = 0.8042), E17.5 (P = 0.0163), and P2 (P = 0.0119). Samples: Counts combined from three brain slices in embryonic or postnatal brains with n = 5, 6 at E15.5; n = 6, 6 at E17.5; n = 6, 6 at P2 for control and PLX5622, respectively. Graphs: Box and Whisker plots with middle line representing median, cross representing mean, box extending from the 25th to 75th percentiles, and whiskers at max and min values. Statistics: Student’s t test, *P ≤ 0.05