Fig. 8

Cytokine profile of slice culture media taken from control and PLX5622 embryonic mouse brain slices of the tuberal hypothalamus. a Heatmap of an array of cytokine, chemokine, and growth factor protein levels (pg/mL) from slice culture media. Samples marked with grey boxes were removed as being statistical outliers when analyzed with Dixon’s Q test with 95% confidence. Select chemokines b CCL2 (P= 0.0085), CXCL1 (P = 0.0211), CXCL2 (P = 0.0193), CXCL10 (P = 0.0034), CX3CL1 (P ≤ 0.0001), IGF-1 (P = 0.0077), c CCL3 (P = 0.0008), and CCL4 (P = 0.0010) found to be significantly different between control and PLX5622 treated samples. Samples: Culture media samples from n = 6 brain slices from separate embryos for both control and PLX5622, except in CXCL1 where n = 5 for control. Graphs: Box and Whisker plots with middle line representing median, cross representing mean, box extending from the 25th to 75th percentiles, and whiskers at max and min values. Statistics: Welch’s t test, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001