Fig. 3

IL-20 promoted astrocyte activation in HA cell line. a Expression of IL-20 and its receptors (IL-20R1, IL-20R2, and IL-22R1) in HA cells was analyzed using immunocytochemistry staining. Data from one representative experiment of three independent experiments is shown. b–f HA cells were treated with IL-20 (200 ng/ml), 7E (2 μg/ml), or IL-20 (200 ng/ml) plus 7E (2 μg/ml) for 4 h. Total RNA was isolated and the transcripts of GFAP, TGF-β1, TNF-α, MCP-1, and IL-6 were analyzed using RT-qPCR with specific primers. GAPDH was an internal control. *P < 0.05 compared with the untreated controls (Ctrl). ^#P < 0.05 compared with the IL-20-treated group. Data are expressed as mean ± SD and are representative of three independent experiments performed in triplicate. g, h Cell migration was evaluated using a wound-healing assay for 24 h. Wound closure was quantified by measuring the area remaining to be immigrated using ImageJ software. *P < 0.05 compared with the untreated controls (Ctrl). ^#P < 0.05 compared with the IL-20-treated group. Data are expressed as mean ± SD and are representative of three independent experiments performed in triplicate