Fig. 2

IC100 treatment reduces peripheral immune cell infiltration into the spinal cord following EAE. a Flow cytometric quantification of the leukocyte populations infiltrating into the spinal cord at 35 dpi after EAE. The flow cytometry gating strategy is provided in Supplementary Figure 1. b Representative flow cytometry plots of CD4 and CD8 T cell populations from mice treated with vehicle and IC100 (30 mg/kg). In order to assure that no dendritic cells expressing CD4 or CD8 were included, we identified the CD4⁺ and CD8⁺ T cell populations after gating on the CD45⁺MHCII⁻ population. Results are expressed as average ± SEM of 5 mice/group, *p < 0.05, **p < 0.001, Mann-Whitney t test; ^p < 0.05, one-way ANOVA, Dunnet’s multiple comparisons test