Fig. 4

ATO reduces the number and proportion of CD4⁺ T cells in EAE mice. a Immunohistochemical staining of CD4 was performed in spinal cord. b Analysis of the infiltration of CD4⁺ T cells in a (n = 3). The percentage of CD4⁺ T cells in spleen (c) and peripheral blood (e) were determined by flow cytometry, respectively. d, f Analysis of CD4⁺ T cells in c, e (n = 3). Data are representative of three independent experiments. **p < 0.01 and ****p < 0.0001 vs. the control group; #p < 0.05, ##p < 0.01, and ####p < 0.0001 the EAE+ATO group vs. the EAE group.