Fig. 5

ATO induces CD4⁺ T cell apoptosis in EAE mice. a, b CD4⁺ T cells were isolated from the spleen using the mouse CD4+ T cell Isolation Kit. Annexin-V/PI staining was performed to analyze CD4⁺ T cell apoptosis (n = 3). c Representative TUNEL staining sections of spinal cord. Boxes denote magnified areas. d Analysis of TUNEL-positive cells in c (n = 3). Data are representative of three independent experiments. *p < 0.05 vs. the control group; #p < 0.05 the EAE+ATO group vs. the EAE group