Fig. 7

Effect of STING on SAH-induced neuroinflammation. a-f Representative western blotting images and quantitative analyses of iNOS, cleaved IL-1β, NLRP3, ASC, and caspase-1 p20 expression at 24 h after SAH. g Relative mRNA level of M1 microglia markers genes (CD16, iNOS, IL-1β, IL-6, TNF-α, and MCP-1). h Representative microphotographs demonstrated the morphological changes in microglia (Iba-1, green) and CD16 positive cells. Resting microglia are characterized by small soma, long protrusions, and rod-shaped nuclei (while arrows), while activated microglia are characterized by large soma, short protrusions, and amoeboid morphology (red arrows). i, j The quantitative analysis of morphological activated microglia and CD16-positive microglia. k, l Representative microphotographs and quantitative analysis of iNOS-positive cells (red). m-o Representative microphotographs and quantitative analysis of caspase-1-positive cells (red) and IL-1β-positive cell (green). n = 6 for each group. Data were represented as mean ± SD. *P < 0.05 versus sham group. #P < 0.05 versus SAH + vehicle group