Fig. 8

Pharmacological inhibition of AMPK with compound C abolished the neuroprotective effect of C-176 against SAH at 24 h post-modeling. a-h Representative western blotting images and quantitative analyses of TBK1 phosphorylation, AMPK phosphorylation, and the expression of iNOS, cleaved IL-1β, NLRP3, ASC, and caspase-1 p20 at 24 h after SAH. n = 6. i, j Representative microphotographs and quantitative analysis of TUNEL assay. n = 6. k Relative mRNA level of M1 microglia markers genes (CD16, iNOS, IL-1β, IL-6, TNF-α, and MCP-1). n = 6. l, m The quantification of neurological function. n = 18. Data were represented as mean ± SD. *P < 0.05 versus sham group. #P < 0.05 versus SAH + vehicle1 group. &P < 0.05 versus SAH + C-176 group