Table 5 Effects of microglial depletion by diphtheria toxin on the effects of LPS on gene expression of markers of microglia/monocytes and proinflammatory cytokines in the brain (hypothalamus) and liver of Cx3cr1-Dtr rats. F values from 2 (diphtheria toxin (DT) vs. control) × 2 (LPS vs. control) ANOVA with 4-8 rats/group. Liver expression of IL-6 was undetectable in saline-treated wild-type and Cx3cr1-Dtr rats. LPS-treated groups were therefore compared by a Student unpaired t test
From: Microglia depletion fails to abrogate inflammation-induced sickness in mice and rats
Target molecule | DT | LPS | DT × LPS |
|---|---|---|---|
Brain Cx3cr1 | F(1, 23) = 124*** | F(1, 23) = 1.14 NS | F(1,23) = 0.16 NS |
Brain Il1b | F(1, 22) = 4.05 NS | F(1, 22) = 5.63* | F(1, 22) = 5.47* |
Brain Tnf | F(1, 24) = 13.8** | F(1, 24) = 2.78 NS | F(1, 24) = 6.17* |
Brain Il6 | F(1, 24) = 0.46 NS | F(1, 24) = 10.64** | F(1, 24) = 2.10 NS |
Brain Il10 | F(1, 22) = 1.55 NS | F(1, 22) = 8.40** | F(1, 22) = 2.27 NS |
Brain Oas1a | F(1, 22) = 4.31* | F(1, 22) = 1.20 NS | F(1, 22) = 0.4 NS |
Liver Cx3cr1 | F(1, 13) = 14.2** | F(1, 13) = 8.42* | F(1, 13) = 4.58+ |
Liver Il1b | F(1, 13) = 7.45* | F(1, 13) = 7.78* | F(1, 13) = 0.79 NS |
Liver Tnf | F(1, 13) = 9.35** | F(1, 13) = 36.4*** | F(1, 13) = 11.7** |
Liver Il6 | Â | Â | t(6) = 3.19* |
Liver Il10 | F(1, 13) = 0.82 NS | F(1, 13) = 1.02 NS | F(1, 13) = 1.12 NS |
Liver Oas1a | F(1, 13) = 1.15 NS | F(1, 13) = 0.92 NS | F(1, 13) = 2.43 NS |