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Table 5 Effects of microglial depletion by diphtheria toxin on the effects of LPS on gene expression of markers of microglia/monocytes and proinflammatory cytokines in the brain (hypothalamus) and liver of Cx3cr1-Dtr rats. F values from 2 (diphtheria toxin (DT) vs. control) × 2 (LPS vs. control) ANOVA with 4-8 rats/group. Liver expression of IL-6 was undetectable in saline-treated wild-type and Cx3cr1-Dtr rats. LPS-treated groups were therefore compared by a Student unpaired t test

From: Microglia depletion fails to abrogate inflammation-induced sickness in mice and rats

Target molecule DT LPS DT × LPS
Brain Cx3cr1 F(1, 23) = 124*** F(1, 23) = 1.14 NS F(1,23) = 0.16 NS
Brain Il1b F(1, 22) = 4.05 NS F(1, 22) = 5.63* F(1, 22) = 5.47*
Brain Tnf F(1, 24) = 13.8** F(1, 24) = 2.78 NS F(1, 24) = 6.17*
Brain Il6 F(1, 24) = 0.46 NS F(1, 24) = 10.64** F(1, 24) = 2.10 NS
Brain Il10 F(1, 22) = 1.55 NS F(1, 22) = 8.40** F(1, 22) = 2.27 NS
Brain Oas1a F(1, 22) = 4.31* F(1, 22) = 1.20 NS F(1, 22) = 0.4 NS
Liver Cx3cr1 F(1, 13) = 14.2** F(1, 13) = 8.42* F(1, 13) = 4.58+
Liver Il1b F(1, 13) = 7.45* F(1, 13) = 7.78* F(1, 13) = 0.79 NS
Liver Tnf F(1, 13) = 9.35** F(1, 13) = 36.4*** F(1, 13) = 11.7**
Liver Il6    t(6) = 3.19*
Liver Il10 F(1, 13) = 0.82 NS F(1, 13) = 1.02 NS F(1, 13) = 1.12 NS
Liver Oas1a F(1, 13) = 1.15 NS F(1, 13) = 0.92 NS F(1, 13) = 2.43 NS
  1. NS non-significant
  2. +p < 0.10, *p < 0.05, **p < 0.01, ***p < 0.001