Fig. 5

Increased mast cell (MC) numbers in the gut mucosa measured in the intestinal samples of young and aged mice for 6 h, 24 h, and 7 days post-stroke compared to the age matched controls. a MCs quantified by immunohistochemistry in the intestinal samples of young and aged mice at 6-h and 24-h post-stroke compared to their age-matched sham controls. b MCs quantified by fluorescence immunohistochemistry in the intestinal samples of aged mice at 7-days post-stroke and c mRNA levels of HR2 quantified by RNA in situ hybridization and analyzed by image-J in the intestine of Ag mice at 7-days post-stroke. n = 3 (sham) and 5 (stroke) per group (a) and 5 (sham) and 5 (stroke) per group (b). Data are expressed as mean ± SEM (a) and (b), as well as individual values. *P < 0.05, **P < 0.01, ***P < 0.001. P values were calculated using one-way analysis of variance with Tukey multiple comparisons correction (a) and two-tailed unpaired Student’s t test (b). a Red arrows-Mast cell; b Red-mast cells