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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Expression profiling of spinal cord dorsal horn in a rat model of complex regional pain syndrome type-I uncovers potential mechanisms mediating pain and neuroinflammation responses

Fig. 9

Pharmacological blocking NLRP3 inflammasome activation attenuated the mechanical allodynia of CPIP rats. a Schematic protocol illustrating the time points for model establishment, behavioral tests, and MCC950 (30 μg/rat in 12.5 μl injection volume, via intrathecal catheter)/vehicle (0.1% DMSO in PBS) application. b NLRP3 expression in ipsilateral SCDH of Sham+Veh, CPIP+MCC950, and CPIP+Veh groups measure by Western blot. Upper panel indicates representative images of NLRP3 and β-actin protein expression. Lower panel indicates summarized NLRP3 expression normalized to β-actin. c IL-1β expression in ipsilateral SCDH of Sham+Veh, CPIP+MCC950, and CPIP+Veh groups measure by Western blot. Upper panel indicates representative images of IL-1β and β-actin protein expression. Lower panel indicates summarized IL-1β expression normalized to β-actin. n = 4 rats/group. d Time course effect of MCC950 on 50% paw withdraw threshold (PWT) of ipsilateral hind paw of CPIP rats. e Summary of the normalized area under the curve (AUC) as in d. n = 6 rats/group. **p < 0.01 vs. Sham+Veh group. ##p < 0.01 vs. CPIP+Veh group. Two-way ANOVA followed by Tukey’s post hoc test was used for comparison in panel d. One-way ANOVA followed by Tukey’s post hoc test was used for comparison in panel e

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