Fig. 1

CD11b expression in cerebral cortical and cerebellar microglias incubated with OMS + NB sera or IgG. Note that the concentration of CD11b, a marker of microglial activation, was upregulated in cerebral cortical microglias (a) and cerebellar microglias (b) incubated with sera from children with OMS and NB (OMS + NB) compared with those from children with NB or healthy children. The IgG fraction (c, d) of the OMS + NB group also elevated the level of CD11b, but not the IgG-free fraction (e, f). No alteration was observed in the expression of CD11b after treatment with commercially available human IgG, the IgG fraction from children with juvenile idiopathic arthritis (JIA) or anti-NMDAR encephalitis (c, d). The expression of CD11b in the cell membrane of microglias was upregulated by IgG of the OMS + NB group (g, h). ^***p < 0.001, one-way ANOVA, n = 10 (health control), n = 20 (NB), n = 10 (OMS + NB), n = 15 (IgG), n = 10 (JIA), n = 6 (anti-NMDAR encephalitis). n = 10/ group (g, h, Western blot)