Fig. 5
From: Function of astrocyte MyD88 in high-fat-diet-induced hypothalamic inflammation
Astrocyte-specific Myd88 KO affects HFD-induced obesity. Astrocyte-specific Myd88 KO (Myd88△GFAP) mice and control Myd88fl/fl mice were fed an HFD for 16 weeks, and then their metabolic parameters were measured. a, b Average daily food intake (a) and average daily calorie intake (b) were measured for a week at 23–24 weeks of age during HFD feeding (n = 4/group). c The accumulated weight gain of the mice was observed every week during HFD feeding (n = 4/group). HFD, high-fat diet. d The weight of adipose tissues was measured at 24 weeks of age (n = 4/group). BAT, brown adipose tissue. e–h Glucose tolerance testing (e, f) and insulin tolerance testing (g, h) were carried out in mice after HFD feeding for 16 weeks (n = 5–9/group). AUC, area under curve. i–m Indirect calorimetry measurements were performed in metabolic cages to determine changes in the oxygen consumption (VO2) (i, j), carbon dioxide generation (VCO2) (k, l), and energy expenditure (m) of mice after HFD feeding for 16 weeks (n = 8/group). Data are presented as mean ± SEM. *p < 0.05, and **p < 0.01