Fig. 4
From: NLRP1 inflammasome contributes to chronic stress-induced depressive-like behaviors in mice
Nlrp1a knockdown blocks NLRP1 inflammasome activation in mice with stress stimuli. a Representative immunoreactive bands showing the protein levels of hippocampal ASC in the control, stress, control shRNA + stress, and Nlrp1a shRNA + stress mice. b Statistical results show that chronic stress increased the protein expression of ASC in hippocampus, while Nlrp1a knockdown inhibited the effects in CUMS (n = 4, F3,12 = 9.88, **P < 0.01 vs control, #P < 0.05 vs CUMS and control shRNA), CRS (n = 4, F3,12 = 8.10, *P < 0.05 vs control, #P < 0.05 vs CRS and control shRNA), RSDS (n = 4, F3,12 = 14.4, **P<0.01 vs control, ##P < 0.01 vs RSDS and control shRNA), and CSDS (n = 4, F3,12 = 15.0, **P < 0.01, ***P < 0.001 vs control, ##P < 0.01 vs CSDS and control shRNA) mice. c Representative immunoreactive bands showing the protein levels of hippocampal caspase-1 in the control, stress, control shRNA + stress, and Nlrp1a shRNA + stress mice. d Statistical results show that chronic stress increased the protein expression of caspase-1 in hippocampus, while Nlrp1a knockdown inhibited the effects in CUMS (n = 4, F3,12 = 8.84, *P < 0.05 vs control, #P < 0.05 vs CUMS and control shRNA), CRS (n = 4, F3,12 = 12.3, **P < 0.01 vs control, ##P < 0.01 vs CRS and control shRNA), RSDS (n = 4, F3,12 = 10.2, **P < 0.01 vs control, #P < 0.05 vs RSDS and control shRNA), and CSDS (n = 4, F3,12 = 8.71, *P < 0.05 vs control, #P < 0.05 vs CSDS and control shRNA) mice. e Statistical results show that chronic stress increased the level of IL-1β in hippocampus, while Nlrp1a knockdown inhibited the effects in CUMS (n = 4, F3,12 = 22.5, ***P < 0.001 vs control, ##P<0.01 vs CUMS and control shRNA), CRS (n = 4, F3,12 = 20.2, **P < 0.01, ***P < 0.001 vs control, ##P < 0.01 vs CRS and control shRNA), RSDS (n = 4, F3,12 = 15.3, **P < 0.01 vs control, ##P < 0.01 vs RSDS and control shRNA) and CSDS (n = 4, F3,12 = 57.5, ***P < 0.001 vs control, ##P < 0.01 vs CSDS and control shRNA) mice. f Statistical results show that chronic stress increased the level of IL-18 in hippocampus, while Nlrp1a knockdown inhibited the effects in CUMS (n = 4, F3,12 = 21.5, **P < 0.01, ***P < 0.001 vs control, ##P < 0.01 vs CUMS and control shRNA), CRS (n = 4, F3,12 = 51.5, **P < 0.01, ***P < 0.001 vs control, ##P < 0.01 vs CRS and control shRNA), RSDS (n = 4, F3,12 = 50.4, **P < 0.01, ***P < 0.001 vs control, ##P < 0.01 vs RSDS and control shRNA), and CSDS (n = 4, F3,12 = 79.7, ***P < 0.001 vs control, ##P < 0.01 vs CSDS and control shRNA) mice. Stress: CUMS, CRS, RSDS, and CSDS. Data are expressed as means ± SEM. One-way ANOVA, Bonferroni test.