Fig. 4

TDAG8 gene deletion reduces the number of satellite glial cells (SGCs) and inhibition of SGCs attenuates chronic phase of RA pain. a, b DRG from TDAG8^+/+ or TDAG8^−/− RA mice were taken at 0, 1, 4, 8, and 12 weeks after RA induction, then co-immunostained with anti-GFAP and anti-PERI antibodies. a Cell images show GFAP- (green) or PERI (red)-positive neurons. Scale bar is 50 μm. b Histograms represent the mean±SEM percentage of PERI-positive neurons surrounded by GFAP⁺ cells (PERI^GFAP+) to total PERI-positive (PERI_T) neurons. c DL-fluorocitric acid (FC) (0.1 nmol) or vehicle was intrathecally injected at week 3 in RA mice, followed by mechanical tests. Data are mean ± SEM of total tested mice (n = 6 per group). ***p < 0.001, FC vs vehicle-treated mice by two-way ANOVA