Fig. 2

FA and FB inhibited the migration of neutrophils to the injury site in CuSO₄-treated zebrafish. a A general view of the zebrafish larvae at 3 dpf. The boxed area represented the horizontal lateral line, and neutrophils within the horizontal line (dotted red lines) were calculated in quantification experiments. b The area of the posterior blood island or caudal hematopoietic tissues (the boxed region with dotted yellow lines) in an untreated transgenic (mpx: EGFP) zebrafish. Most leukocytes in this development stage were distributed in this area. c Microphotographs exhibited neutrophils migration in the control, model, FA, and FB groups (neutrophils of 3 dpf transgenic (mpx: EGFP) zebrafish exhibiting green fluorescence). The red dotted lines represented the location of the horizontal muscles of zebrafish. d FA and FB dose-dependently reduced the number of neutrophils recruited in the injury site. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, compared with the control group; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, compared with the model group. Data were shown as mean ± S.D, n = 35