Fig. 1

An HSD has no effect on body weight and food intake but does increase water consumption, which affects mouse plasma and urine osmolality. a The bodyweights (g) of male/female mice on HSD and ND were recorded for 3 months, n = 10–14/group. No significant difference was observed between HSD and ND fed mice. b High-salt and regular laboratory chow intake (g) was measured, n = 10–12/group. No significant difference was observed between HSD and ND fed mice. c Average distilled water and 1% NaCl water (g) consumption were recorded on a weekly basis, n = 10–12/group. Mice on an HSD drunk significantly more water than mice on a ND. Male and female mice plasma (d) and urine (e) osmolality (mOsm/kg) was monitored at 1 month and 3 months during special diet feeding, n = 3–4/group. All data were presented as mean ± SEM, and data was analyzed with unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001