Fig. 7
An HSD failed in inducing myeloid cell activation and enhancing pain sensitivity in CCR2 KO mice. a WT and CCRKO mice on a ND have similar mechanical thresholds. While 2 months HSD reduced paw withdrawal thresholds to mechanical stimuli in WT mice, it failed to do so in CCR2KO mice, n = 6–11/group. b When compared to WT mice, CCR2KO mice had significantly lower numbers of circulating monocytes, and only a slight increase was found when challenged with an HSD. The Ly6Chi proinflammatory subset was significantly reduced in CCR2 KO mice and there was a slight increase in HSD mice. N = 6–11/group. c An HSD was not able to significantly increase the number of CD11b+F4/80+ macrophages in the peripheral nerves of CCR2KO mice. N = 6–11/group. d An HSD was not able to increase the number of Iba-1+ microglia in the lumbar spinal cord of CCR2KO mice. N = 6/group. Scale bar: 50 μm. The outline indicates the area of interest (lamina I–III) where microglia number was quantified. No significant difference was observed between male and female mice; therefore, the data was combined. All data was presented as mean ± SEM and analyzed with an unpaired t test. *p < 0.05, ***p < 0.001