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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Evaluation of M2-like macrophage enrichment after diffuse traumatic brain injury through transient interleukin-4 expression from engineered mesenchymal stromal cells

Fig. 1

Transfection of MSCs and protein expression. a GFP fluorescence intensity after MSCs are transfected with naked GFP mRNA or complexed with one of three vectors. b Viability of MSCs with increasing dosage of synthetic IL-4 mRNA complexed with Viromer Red. c Concentration of IL-4 synthesized in media with IL-4 mRNA-transfected MSCs. d MSCs do not endogenously synthesize IL-4 and require transfection with IL-4 mRNA. e Concentration of IL-4 synthesized over 48 h from IL-4 mRNA-transfected MSCs. Mean ± SD, one-way ANOVAs with Tukey’s post hoc test, and Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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Journal of Neuroinflammation

ISSN: 1742-2094