Fig. 3

MSC transfection characterization for in vivo delivery. a Concentration of IL-4 in media of MSCs transfected with synthetic IL-4 mRNA complexed with Viromer Red. The complexes were incubated with the MSCs for varying amounts of time (0–24 h). Then, the media was sampled 24 h after each time point for IL-4 quantification via ELISA. b Viability of MSCs while on ice. No significant differences observed. c Concentration of IL-4 synthesized while transfected and harvested MSCs were kept on ice (0–6 h) and then over 24 h after re-plating and kept at 37 °C. d Amount of IL-4 expressed by 300,000 MSCs transfected for 10 h (“prior to harvest”) and then a sample of 150,000 MSCs 24 h after. Mean ± SD and two-way ANOVA comparing between groups with Tukey’s post hoc; *p < 0.05