Fig. 5
From: Rh-CSF1 attenuates neuroinflammation via the CSF1R/PLCG2/PKCε pathway in a rat model of neonatal HIE
Effects of BLZ945 and U73122 co-administration with rh-CSF1 on immunofluorescence staining of MPO and IL-1β at 48 h after HI. Representative microphotographs (a-b) and quantification of (c-d) IL-1β and MPO positive cells showed the number of IL-1β/MPO-positive cells was significantly increased in all HI animals compared to shams. Rh-CSF1 treatment or Rh-CSF1 with DMSO significantly reduced the neuroinflammation, but such effects were reversed by either BLZ945 or U73122. *P < 0.05 vs. sham; #P < 0.05 vs. HI+ vehicle; @P < 0.05 vs. HI+ rh-CSF1 + DMSO; Scale bar =100 μm; Data are represented as mean ± SD, n = 4 in each group