Fig. 5

Increased number of IBA1-immunoreactive and GFAP-immunoreactive cells in the SNpC of 18-month-old animals. a Representative histologic sections of the SNpC obtained from 1- and 18-month-old animals stained with anti-IBA1 antibodies and counterstained with BOND polymer detection. b IBA1-immunoreactive cells were quantified (10 fields/animal, n = 3). The number of IBA1-immunoreactive cells was significantly greater in 18-month-old animals compared to 1-month-old animals, indicating number of microglia in the SNpC increases with age. (Nlrp3^WT ** p = 0.0094, one-way ANOVA; Nlrp3^A350V ** p = 0.0041, one-way ANOVA). c Representative IHC images of SNpC tissues stained with anti-GFAP antibodies followed by BOND polymer detection. d GFAP-immunoreactive cells were quantified (10 fields/animal, n = 4). GFAP-immunoreactive cell number increases with age in all cohorts (Nlrp3^WT * p = 0.03, Nlrp3^A350V * p = 0.01, Nlrp3^L351P *** p = 0.0008, one-way ANOVA). The number of GFAP-immunoreactive cells was significantly greater in 18-month-old Nlrp3^L351P animals compared to the number of cells in 18-month-old Nlrp3^WT and Nlrp3^A350V animals (** p = 0.004 and * p = 0.01 respectively, one-way ANOVA). Scale bars represent 20 μM. Error bars represent s.e.m.