Fig. 1

Decreased expression of p-AMPK and αSMA in human unruptured and ruptured IA tissue compared to STA. STA and IA were double-labeled with antibodies against α-SMA (red) and p-AMPK (green) and stained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Compared to STA, p-AMPK and α-SMA expression was lower in unruptured and ruptured IA vessel walls. a Representative immunofluorescence images of human tissues. b, c Quantitative analysis of cells positive for α-SMA (b) and p-AMPK (c). *P < 0.05 vs STA. ^#P < 0.05 vs ruptured IA. Scale bar = 20 μM