Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Metformin inhibits intracranial aneurysm formation and progression by regulating vascular smooth muscle cell phenotype switching via the AMPK/ACC pathway

Fig. 3

Metformin inhibits p-AMPK and contractile marker expression in VSMCs in a rat IA model. A Representative images and quantitative analysis of α-SMA (red) and p-AMPK (green) expression in rat IAs in control, metformin, compound C, and metformin+ compound C groups; nuclei were counterstained with 4′,6-diamidino-2-phenylindole DAPI (blue). Metformin increased the expression of α-SMA and p-AMPK. (a) Representative images of immunofluorescence in rat IA sections. (b) Quantitative analysis of p-AMPK-positive VSMCs. B Representative images and quantitative analysis of α-SMA (red) and SM22α (green) immunofluorescence in VSMCs. Metformin enhanced the expression of both markers. (a) Representative images of immunofluorescence labeling of rat IA sections. (b, c) Quantitative analysis of α-SMA-positive (b) and SM22α-positive (c) cells. #P < 0.05 vs 500 μM metformin group. Scale bar = 50 μM

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094