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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: 25-Hydroxycholesterol amplifies microglial IL-1β production in an apoE isoform-dependent manner

Fig. 2

LPS stimulates 25HC production and CH25H expression in primary microglia and in mouse brain. a LPS stimulates 25-HC production and secretion in primary microglia in a time- and dose-dependent manner. Primary microglia were treated with LPS (0, 10, and 100 ng/ml) for 6 and 24 h. The levels of 25-HC in cells (a) and media (b) were determined by GC-MS. ****p < 0.001 by ordinary one-way ANOVA. c LPS induces the expression of CH25H, IL-1β, TNFα, and NLRP3 inflammasome mRNA in primary microglia. The comparative gene expressions were determined by qPCR using RNA extracted from primary microglia with or without 10 ng/ml LPS treatment for 24 h. Insert: CH25H protein level in CH25H+/+ or CH25H-/- primary microglia treated with or without 10 ng/ml LPS. It is a representative result of two independent experiments. d Gene expression analysis of CH25H, IL-1β, TNFα, Cyp7b, and Cyp27a1 in brain tissue of C57BL6 mice treated with 8.2 mg/kg LPS (n = 3) for 24 h as determined by qPCR. *p < 0.05,**p < 0.01, ***p < 0.005 by Student t test comparing LPS-treated mouse brain to saline-treated control brain

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