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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Intracerebral overexpression of miR-669c is protective in mouse ischemic stroke model by targeting MyD88 and inducing alternative microglial/macrophage activation

Fig. 1

MiR-669c-3p expression is increased under conditions of OGD/R and ischemia. Quantitative real-time PCR for miR-669c-3p in N2a cells exposed to oxygen and glucose deprivation (OGD) for either 1, 2, or 3 h followed by reoxygenation for additional 24 h. a One-way ANOVA followed by Bonferroni’s post hoc tests, ***p < 0.001 compared to normoxic control cells (CTRL), N = 3-10. MiR-669c-3p expression is increased in primary cortical neurons exposed to excitotoxic injury (b). Quantitative real-time PCR for miR-669c-3p in primary cortical neurons treated with 400 μM glutamate (GLU) for 24 h. Unpaired two-tailed t test: **p < 0.01 compared to vehicle-treated primary cortical neurons (CTRL), N = 3-5. MiR-669c overexpression does not prevent the OGD/R-induced neuronal death in N2a cells (c). MTT assay of N2a cells exposed to OGD for 2 h followed by reoxygenation for 24 h. OGD/R reduced cell viability by approximately 40% as compared to normoxic cells. The assay was repeated three times with similar results. One-way ANOVA followed by Bonferroni’s post hoc tests: ***p < 0.001 or ###p < 0.001 compared to respectively LV1-GFP (GFP) and LV1-miR-669c (669) transduced normoxic cells (CTRL), N = 10-11. MiR-669c-3p expression is increased in LV1-miR-669c transduced N2a cells (d). Quantitative real-time PCR for miR-669c-3p in N2a cells transduced either with control LV1-GFP (GFP) or LV1-miR-669c (669). Unpaired two-tailed t test: ***p < 0.001 compared to LV1-GFP transduced cells, N = 6 in each group. Quantitative real-time PCR for miR-669c-3p in contralateral cortex (CONTRA) and peri-ischemic cortex area of the ipsilateral hemisphere (IPSI) at 1 (e) and 3 dpi (f) after pMCAo. Paired two-tailed t tests: **p < 0.01 compared to contralateral hemisphere, N = 6-8. Quantitative real-time PCR for miR-669c-3p in contralateral hemisphere (CONTRA) and ipsilateral hemisphere (IPSI) at 1 (g) and 3 dpi (h) after tMCAo. Paired two-tailed t tests, N = 3

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