Fig. 2

MiR-669c overexpression modulates BV2 microglial phenotype under LPS-induced inflammation. The expression of Iba1, CX3CR1, as well pro- and anti-inflammatory mediators MMP9, TNF-α, IL-6, IL-1β, CCL2, Arg1, Chil3/Ym1, PPAR-γ, IL-10, and TGF-β were analyzed by quantitative real-time PCR in LV1-GFP (control) and LV1-miR-669c transduced cells exposed to LPS (50 ng/ml) for 24 h. The assay was repeated three times with similar results. Values for miR-669c overexpressing cells normalized to LV1-GFP transduced (control) cells, presented as a solid line on graph. Unpaired two-tailed t tests: *p < 0.05, **p < 0.01, ***p < 0.001 compared to LV1-GFP transduced BV2 cells. N = 3 in each group