Fig. 1

A1-specific transcripts were induced in astrocytes. a Representative graph showing the relative mRNA levels of the indicated factors from astrocytes isolated from neonatal mice (top). Immunofluorescent staining for GFAP and IBA1 on cultured astrocytes (bottom). b Representative graph showing the relative mRNA levels of the indicated factors. Astrocytes were either untreated or treated with indicated doses of LPS (100 ng/mL, 1000 ng/mL), TNFα (30 ng/mL), IL-1α (10 ng/mL) plus TNFα (10 ng/mL, 30 ng/mL, 50 ng/mL), TGFβ (3 ng/mL), FGF (20 ng/mL), or IL-1β (30 ng/mL) for 24 h and then collected for quantitative RT-PCR. Data shown are means ± SD of two independent experiments. *p < 0.05 and **p < 0.01