Fig. 13

Comparison of the representative taxonomic abundance among different groups. a Linear discriminant analysis (LDA) effect size (LEfSe) analysis revealed significant bacterial differences in fecal microbiota between different groups. The LDA scores (log10) > 4 and P < 0.05 are listed. b Cladogram using LEfSe method indicating the phylogenetic distribution of fecal microbiota associated with different groups. Metastats analysis indicated the significant differences in Roseburia between two groups of APP/PS1 group and APP/PS1 27-OHC group (c). Metastats analysis indicated the significant differences in Roseburia between two groups of APP/PS1 27-OHC group and APP/PS1 ANS group (b). p phylum, c class, o order, f family, g genus. Groups: WT: wild-type control group of C57BL/6J mice; APP/PS1 Con: transgenic control group of APP/PS1 mice; WT 27-OHC: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 27-OHC: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol; APP/PS1 ANS: APP/PS1 mice treated with 0.2 mg/day anastrozole; WT CO: C57BL/6J mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole; APP/PS1 CO: APP/PS1 mice treated with 5.5 mg/kg 27-hydroxycholesterol plus 0.2 mg/day anastrozole. n = 6/group