Fig. 3

Effects of ethanol on the density of BLBP⁺ radial glia in NEP and mHYP. Maternal ethanol administration group (2 g/kg/day, E10–E15) was compared to Untreated and isocaloric Control groups of E19 embryos, and the density of cells and processes was examined using single-labeled immunofluorescence histochemistry. a Ethanol significantly increased the density of BLBP⁺ radial glia cells in NEP and of BLBP⁺ processes in mHYP of both female and male embryos, with females showing a significantly greater effect. b These effects in ethanol-exposed compared to control embryos are illustrated in representative immunostaining images (× 20) of single-labeled BLBP⁺ cells in NEP and BLBP⁺ processes in mHYP of female and male embryos. Data are mean ± SEM. (n = 7/group/sex, *p < 0.05 versus control group, ^#p < 0.05 versus males). Two-way ANOVA was used to compare means between groups, simple main effect analyses to test the differences between sexes as well as differences within each sex, and paired t tests to directly compare the effects within each sex of maternal treatment vs control groups. BLBP, brain lipid-binding protein; mHYP, medial hypothalamus; NEP, neuroepithelium; V, third ventricle. Scale bars, 200 μm