Fig. 5

Stimulatory effects of CCL2 injection on BLBP⁺ and CCL2⁺ cells and processes in NEP and mHYP. Maternal administration of CCL2 (4 μg/kg/day, E10–E15) group was compared to Untreated and vehicle Control groups of female embryos at E19, and the density of cells and processes was analyzed using single-labeled immunofluorescence histochemistry. a CCL2 administration compared to control groups significantly increased the density of BLBP⁺ and CCL2⁺ cells in NEP and processes in mHYP. b These effects of ethanol compared to isocaloric Control group are illustrated in representative immunostaining images of double-labeled BLBP⁺ cells and processes (red) and CCL2⁺ cells and processes (green) with the nuclear stain DAPI (blue). Data are mean ± SEM. (n = 7/group, *p < 0.05 versus control group). One-way ANOVA was used to compare group means followed by SDS post hoc test. mHYP, medial hypothalamus; NEP, neuroepithelium; V, third ventricle. Scale bar, 100 μm