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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons

Fig. 6

Impact of CCR2 receptor antagonist on ethanol’s effects on BLBP+ and CCL2+ cells and processes. Maternal injection of CCR2 receptor antagonist, INCB3344 (1 mg/kg/day, i.p.), was performed during the period of maternal ethanol administration (2 g/kg/day, E10–E15) and was compared to the Ethanol, Control, and Control + INCB3344 groups of E19 female embryos. a Analysis using single-labeled immunofluorescence histochemistry showed that Ethanol treatment compared to Control group significantly increased the density of BLBP+ and CCL2+ cells in NEP and processes in mHYP and the INCB3344 treatment during ethanol exposure blocked this effect, causing a significant decrease in the Ethanol + INCB3344 group compared to Ethanol group in cells and processes reduced to the same levels as those in the Control group. b These effects in the embryo are illustrated in representative images (× 20) of BLBP+ and CCL2+ single-labeled cells and processes in the Control, Ethanol, and Ethanol + INCB3344 embryos. Data are mean ± SEM (n = 7/group, *p < 0.05 versus the Control, Ethanol + INCB3344, and Control + INCB3344 groups). Differences between groups were analyzed using one-way ANOVA followed by SDS post hoc test. BLBP, brain lipid-binding protein; mHYP, medial hypothalamus; NEP, neuroepithelium; V, third ventricle. Scale bar, 100 μm

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