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Table 3 Antibodies used for double-labeling immunofluorescence histochemistry

From: CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons

Combination Primary antibody Dilution Vendor Secondary antibody Dilution Vendor
CCL2+BLBP Rabbit anti-CCL2 1:200 Biorbyt, CA Bio-Horse anti-Rabbit IgG + TSA Fluorescein 1:100 Vector, CA
Mouse anti-BLBP 1:200 Abcam, MA Cy3-Donkey anti-Mouse IgG 1:100 Jackson ImmunoResearch Laboratories. Inc, PA
CCL2+NeuN Rabbit anti-CCL2 1:200 Biorbyt, CA Bio-Horse anti-Rabbit IgG + TSA Fluorescein 1:100 Vector, CA
Mouse anti-NeuN 1:100 EMD Millipore, MA Cy3-Donkey anti-Mouse IgG 1:100 Jackson ImmunoResearch Laboratories. Inc, PA
CCL2+Iba-1 Rabbit anti-CCL2 1:200 Biorbyt, CA Bio-Horse anti-Rabbit IgG + TSA Fluorescein 1:100 Vector, CA
Goat anti-Iba-1 1:200 Abcam, MA Cy3-Donkey anti-Goat IgG 1:100 Jackson ImmunoResearch Laboratories. Inc, PA
MCH+BLBP Rabbit anti-MCH 1:1000 Phoenix Pharmaceuticals, CA FITC-Donkey anti-Rabbit IgG 1:50 Jackson ImmunoResearch Laboratories. Inc, PA
Mouse anti-BLBP 1:200 Abcam, MA Cy3-Donkey anti-Mouse IgG 1:100 Jackson ImmunoResearch Laboratories. Inc, PA
  1. CCL2 C-C motif ligand 2, BLBP radial glia marker brain lipid-binding protein, NeuN neuronal marker, Iba-1 microglia marker, MCH melanin-concentrating hormone