Table 3 Antibodies used for double-labeling immunofluorescence histochemistry
Combination | Primary antibody | Dilution | Vendor | Secondary antibody | Dilution | Vendor |
|---|---|---|---|---|---|---|
CCL2+BLBP | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
Mouse anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
CCL2+NeuN | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
Mouse anti-NeuN | 1:100 | EMD Millipore, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
CCL2+Iba-1 | Rabbit anti-CCL2 | 1:200 | Biorbyt, CA | Bio-Horse anti-Rabbit IgG + TSA Fluorescein | 1:100 | Vector, CA |
Goat anti-Iba-1 | 1:200 | Abcam, MA | Cy3-Donkey anti-Goat IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA | |
MCH+BLBP | Rabbit anti-MCH | 1:1000 | Phoenix Pharmaceuticals, CA | FITC-Donkey anti-Rabbit IgG | 1:50 | Jackson ImmunoResearch Laboratories. Inc, PA |
Mouse anti-BLBP | 1:200 | Abcam, MA | Cy3-Donkey anti-Mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories. Inc, PA |