CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons

Table 5 Effects of maternal ethanol administration on mRNA of CCL2 and CCR2 in NEP+mHYP area in E19 embryos

mRNA	Female			Male
Expression	Untreated	Control	Ethanol	Untreated	Control	Ethanol
CCL2	1.2E− 3 ± 5.7E− 5	1.2E− 3 ± 5.1E− 5	1.9E− 3 ± 4.0E− 5*^#	1.3E− 3 ± 6.1E− 5	1.3E− 3 ± 5.5E− 5	1.4E− 3 ± 5.7E− 5
CCR2	4.7E− 3 ± 2.3E− 4	4.5E− 3 ± 2.1E− 4	6.7E− 3 ± 3.1E− 4*^#	4.6E− 3 ± 4.0E− 4	4.2E− 3 ± 2.3E− 4	5.0E− 3 ± 2.1E− 4*

Effect of maternal administration of ethanol (2 g/kg/day, E10–E15) on mRNA levels of CCL2 and CCR2 was measured using qRT-PCR. Data are expressed as an averaged ratio (target gene expression/house-keeping gene expression) in each group and presented as mean ± SEM (n = 7/group/sex, *p < 0.05 versus Control). Two-way ANOVA was used to compare means between groups, simple main effect analyses to test differences between sexes as well as differences within each sex, and paired t tests to directly compare within each sex the effects of maternal treatment vs control groups
NEP hypothalamic neuroepithelium, mHYP medial hypothalamus, qRT-PCR real-time quantitative PCR
^#p < 0.05 vs males

ISSN: 1742-2094