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Table 5 Effects of maternal ethanol administration on mRNA of CCL2 and CCR2 in NEP+mHYP area in E19 embryos

From: CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons

mRNA Female Male
Expression Untreated Control Ethanol Untreated Control Ethanol
CCL2 1.2E− 3 ± 5.7E− 5 1.2E− 3 ± 5.1E− 5 1.9E− 3 ± 4.0E− 5*# 1.3E− 3 ± 6.1E− 5 1.3E− 3 ± 5.5E− 5 1.4E− 3 ± 5.7E− 5
CCR2 4.7E− 3 ± 2.3E− 4 4.5E− 3 ± 2.1E− 4 6.7E− 3 ± 3.1E− 4*# 4.6E− 3 ± 4.0E− 4 4.2E− 3 ± 2.3E− 4 5.0E− 3 ± 2.1E− 4*
  1. Effect of maternal administration of ethanol (2 g/kg/day, E10–E15) on mRNA levels of CCL2 and CCR2 was measured using qRT-PCR. Data are expressed as an averaged ratio (target gene expression/house-keeping gene expression) in each group and presented as mean ± SEM (n = 7/group/sex, *p < 0.05 versus Control). Two-way ANOVA was used to compare means between groups, simple main effect analyses to test differences between sexes as well as differences within each sex, and paired t tests to directly compare within each sex the effects of maternal treatment vs control groups
  2. NEP hypothalamic neuroepithelium, mHYP medial hypothalamus, qRT-PCR real-time quantitative PCR
  3. #p < 0.05 vs males