CCL2/CCR2 system in neuroepithelial radial glia progenitor cells: involvement in stimulatory, sexually dimorphic effects of maternal ethanol on embryonic development of hypothalamic peptide neurons

Table 7 Effects of ethanol on density of cells or processes that label CCL2, BLBP, NeuN, or Iba-1 in NEP and mHYP

Labeled cells/processes	Location	Control (cells, objects/μm²)	Ethanol (cells, objects/μm²)
CCL2⁺	NEP	6.30E− 5 ± 2.67E− 6	7.50E− 5 ± 2.07E− 6*
CCL2⁺	mHYP	9.90E− 5 ± 7.30E− 6	1.70E− 4 ± 1.46E− 5*
BLBP⁺	NEP	1.51E− 4 ± 4.62E− 6	1.83E− 4 ± 5.67E− 6*
BLBP⁺	mHYP	1.59E− 4 ± 1.32E− 5	2.31E− 4 ± 2.13E− 5*
NeuN⁺	NEP	5.75E− 5 ± 2.77E− 6	7.92E− 5 ± 7.24E− 6*
NeuN⁺	mHYP	4.28E− 5 ± 2.68E− 6	5.17E− 5 ± 2.64E− 6*
Iba-1⁺	NEP	3.79E− 5 ± 1.84E− 6	4.73E− 5 ± 1.79E− 6*
Iba-1⁺	mHYP	4.47E− 5 ± 2.14 E− 6	6.06E− 5 ± 4.27E− 6*

Maternal administration of ethanol (2 g/kg/day, E10–E15) was compared to an isocaloric maltose-dextrin control solution (Control) group in the NEP and mHYP areas of female E19 embryos, measured using single-labeling IF. Data are mean ± SEM, n = 6/group, *p < 0.05 versus Control. The difference between groups was analyzed using Student t test
BLBP brain lipid-binding protein, CCL2 C-C motif ligand 2, Iba-1 microglial marker, mHYP medial hypothalamus, NEP hypothalamic neuroepithelium, NeuN neuronal marker

ISSN: 1742-2094