Fig. 1

Lipocalin-2(Lcn2) upregulation nonalcoholic steatohepatitis (NASH) brain tissues. a Immunoreactivity (Lcn2) as shown by immunohistochemistry in liver and brain slices from mice fed with CHOW diet serves as a control and MCD diet (NASH). Images were taken at × 20 magnification (Scale 100 μm). b Morphometric analysis of Lcn2 immunoreactivity (mean data measured as arbitrary light units from three separate microscopic fields were plotted on y-axis) in CHOW and MCD fed mouse groups ( ***p < 0.001). C. Serum Lcn2 level in pg/mL was plotted as a bar graph with CHOW and MCD fed mice groups. d mRNA expression of receptors of IL-1, TNFα, and Lcn2 (24p3R) in CHOW and MCD fed mouse groups.e, f Co-localization and morphometric analysis of Lcn2 reactivity with astrocytes (GFAP), microglia (CD11b), brain endothelial cells (CD31)m and total neurons (NeuN)