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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Lipocalin 2 induces neuroinflammation and blood-brain barrier dysfunction through liver-brain axis in murine model of nonalcoholic steatohepatitis

Fig. 3

Upregulated expression of Lcn2 is correlated with neurodegeneration. a Neurodegenerative markers BDNF and phosphorylated Tau protein (Serine 396) were shown by immunohistochemistry in brain slices (focused area is cerebral cortex) from mice fed with CHOW diet serve as a control and MCD diet (NASH). Immunoreactivity was indicated by black arrows. Images were taken × 20 magnification. b Morphometric analysis of BDNF and pTau immunoreactivity (mean data measured as arbitrary light units from three separate microscopic fields were plotted on y-axis) in CHOW and MCD fed mouse groups (***p < 0.001). c mRNA expression analysis of BDNF in cerebral cortex of CHOW and MCD fed mouse groups. mRNA expression was assessed by quantitative real-time PCR, and expression was normalized against CHOW diet group, *p < 0.05, **p < 0.01, ***p < 0.001. d Immunoblot analysis with pTau and Tau proteins from cerebral cortex from CHOW and MCD fed mouse groups. e Densitometric analysis of pTau reactivity calculated as pTau and Tau ratio, displayed as mean ± SD (n = 3) and plotted as bar graph, and statistical significance was tested. f Immunoblot analyses of BDNF from cerebral cortex of CHOW and MCD mouse groups. g Densitometric analyses of BDNF immunoreactivity displayed as mean ± SD (n = 3), normalized against β-actin and plotted as bar graph

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