Fig. 7

Activation of NLRP3 inflammasome in brain tissues of murine MAFLD model. a, b Co-localization and morphometric analysis of NLRP3/ASC and NLRP3/Caspase1 as shown by immunofluorescence imaging in brain sections from CHOW and MCD fed mouse groups. Co-localization was displayed yellow dots, indicated by white circles. Images were taken at × 20. Immunoreactivity of co-localization was displayed by yellow dots in a, and immunoreactivity was displayed as bar graph representing mean ± SD of ROI % of 3 different areas (n = 3). c mRNA expression was assessed by quantitative real-time PCR, and expression was normalized against CHOW diet group, *p < 0.05, **p < 0.01, ***p < 0.001