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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: NLRP3 inflammasome-mediated microglial pyroptosis is critically involved in the development of post-cardiac arrest brain injury

Fig. 2

Cardiac arrest induces microglial activation, microglial pyroptosis and leukocytes infiltration. a, b Representative flow cytometry plots of all CD11b+ cells from sham-operated (n = 5) and post-cardiac arrest (n = 6) brain tissues of the hippocampus and cortex, accompanied by the quantification of the percentages of resting microglia, activated microglia and infiltrating CD11b+ leukocytes. c, d Representative flow cytometry plots and quantification of pyroptotic cells (Q2) and caspase-1+ live cells (Q3) in all microglia, labeled with LIVE/DEAD Fixable Near-IR Dead Cell Stain fluorescent probe and FAM-FLICA fluorescent probe. eh Representative flow cytometry plots and quantification of pyroptotic cells and caspase-1+ live cells in the activated and resting microglia population. All data in this figure are analyzed using parametric test. Data are presented as means ± SD. ***P < 0.001 versus sham group (two-sided Student’s t test). CA/CPR cardiac arrest and cardiopulmonary resuscitation

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