Fig. 6
The effect of MCC950 on microglial pyroptosis and caspase-1 activity. a, b Detection of pyroptotic cells (FLICAhigh, LIVE/DEADhigh) and caspase-1+ live cells (FLICAhigh, LIVE/DEADlow) in all microglia, activated microglia, and resting microglia population, respectively. c, d Detection of infiltrating CD11b+ leukocytes, resting and activated microglia from the cortex and hippocampus of sham, vehicle, and MCC950 groups at 48 h after ROSC. All data in this figure are analyzed using parametric test. Data are presented as mean ± SD. Statistical significances are determined with one-way ANOVA followed by Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham; #P < 0.05, ##P < 0.01, ###P < 0.001 versus vehicle. n = 5–6 per group